Advanced Biotech Molecular Design & Engineering
We offer sophisticated design services for mRNA, DNA, and proteins to ensure molecules are optimized for expression, stability, and manufacturability.
mRNA Drug Substance Design
Bespoke guidance on sequence architecture, including codon optimization, secondary structure engineering to fine-tune ribosome loading, choice of cap, polyA tail design, and UTR engineering. We also provide guidance on modified nucleotide strategies (e.g., N1-methylpseudouridine) to maximize in vivo stability and reduce innate immune response.
DNA Plasmid Engineering
We assist with plasmid backbone selection for high-copy yield, promoter optimization for mRNA (T7, SP6, etc.) or for protein expression (insights into promoter strength v/s tight regulation), linearization strategy, copy number compatibility, selection of E. coli strain for DNA v/s protein manufacturing and the implementation of antibiotic-free selection systems for clinical compliance.
Protein design
We offer guidance based on deep structure based computational expertise on improving protein expression, stability, enzyme catalysis to influence Km, Kcat, activity, and substrate specificity tailored to your goals. We profess utilization of state-of-the art structure guided molecular dynamics to facilitate binding affinity and re-create the target intended environment for the protein to accurately predict outcomes thereby mutant library size to maximize chances of success in the lab. Baked in are strategies to identify available variants in nature and utilization of smart codon choices suited to the target expression host.
Let us help maximize your impact.
Smart molecule design is critical for execution!
Our strategy is built for robustness, success and speed. The correct design strategy saves time and decreases wet lab experiment design space, enables faster synthesis and scale up optimization to accelerate development freeing up your resources. Reduction of royalty and licensing costs and avoid common pitfalls. Reduced COGs and longer-term strategy baked in to ensure compliance with regulatory requirements.
Molecule Design FAQs
What types of molecules do you support?
We specialize in mRNA sequence architecture, DNA plasmids, and complex protein designs, including bespoke enzyme engineering for expression, stability and catalysis.
How do you optimize mRNA expression?
Our comprehensive strategy involves codon optimization, secondary structure engineering to fine-tune ribosome loading, specialized UTR and polyA tail engineering tailored to host expression. All designs inherently bake in reduction of innate immune response, manufacturability considerations and cost reduction.
Can you help with insoluble protein expression?
Yes, we solve expression bottlenecks like inclusion bodies and toxic protein expression through tight promoter management, codon variant analysis, scanning and removal of sensitive surface residues and specialized host strain selection.